• microRNA control of Hox gene networks
  • Genomic/epigenomic regulation of axis elongation and vertebral patterning
  • Formation and patterning of spinal cord circuitry
  • Evolutionary acquisition of microRNAs shapes developmental networks

Developmental gene networks

The accuracy and reproducibility with which the vertebrate embryo develops is remarkable. Our lab investigates how developmental gene networks are regulated, both at a transcriptional and post-transcriptional level, to achieve such exquisite reproducibility. 

Our lab continues to make seminal contributions to the understanding of how microRNA regulatory mechanisms contribute to refining or stabilising Hox functional output in mouse, how this relates to human disease and how differences in microRNA acquisition between species may shape differences in morphology.

In particular, we are interested in how the developmental modules that define total vertebral number are integrated with those that impart vertebral identity. Moreover, our lab has developed novel genetic tools to address critical questions regarding formation and function of neurons within the spinal cord that relay sensory information directly to the brain. 

Fluorescent transgenic reporter in mouse reveals dynamic Hox gene expression boundaries
Expression of miR-10 in the developing snake, Ophiophagus Hannah.
Neural networks of the spinal cord

 

Edwina McGlinn is an EMBL-Australia Partner Network Lab Group Leader, based at the Australian Regenerative Medicine Institute, Monash University. Edwina completed a PhD in developmental and molecular biology (1999-2004) with Associate Professor Carol Wicking at the Institute for Molecular Bioscience UQ, identifying novel downstream effectors of Sonic hedgehog in the developing mouse limb. She then became a research fellow in the laboratory of Professor Clifford Tabin, Harvard Medical School USA (2004-2010), elucidating genetic networks involved in patterning the vertebrate limb and axial skeleton. 

Authors
Title
Published In

Wong SF, Agarwal V, Mansfield JH, Denans N, Schwartz MG, Prosser HM, Pourquié O, Bartel DP, Tabin CJ, McGlinn E.

Independent regulation of vertebral number and vertebral identity by microRNA-196 paralogs.

Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4884-93. doi: 10.1073/pnas.1512655112. Epub 2015 Aug 17.

Vonk FJ, Casewell NR, Henkel CV, Heimberg AM, Jansen HJ, McCleary RJ, Kerkkamp HM, Vos RA, Guerreiro I, Calvete JJ, Wüster W, Woods AE, Logan JM, Harrison RA, Castoe TA, de Koning AP, Pollock DD, Yandell M, Calderon D, Renjifo C, Currier RB, Salgado D, Pla D, Sanz L, Hyder AS, Ribeiro JM, Arntzen JW, van den Thillart GE, Boetzer M, Pirovano W, Dirks RP, Spaink HP, Duboule D, McGlinn E, Kini RM, Richardson MK.

The king cobra genome reveals dynamic gene evolution and adaptation in the snake venom system.

PNAS 2013;110(51):20651-6. doi: 10.1073/pnas.1314702110.

Heimberg A, McGlinn E.

Building a robust a-p axis.

Curr Genomics. 2012 Jun;13(4):278-88. doi: 10.2174/138920212800793348.

McGlinn E, Yekta S, Mansfield JH, Soutschek J, Bartel DP, Tabin CJ.

In ovo application of antagomiRs indicates a role for miR-196 in patterning the chick axial skeleton through Hox gene regulation.

Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18610-5. doi: 10.1073/pnas.0910374106. Epub 2009 Oct 21.

Mao J*, McGlinn E*, Huang P, Tabin CJ, McMahon AP.

Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb.

Dev Cell. 2009 Apr;16(4):600-6. doi: 10.1016/j.devcel.2009.02.005.