Investigating protein networks and mechanisms that regulate protein trafficking, signalling and organelle function
The Apaja Group investigates the protein networks and molecular mechanisms that regulate membrane trafficking and proteostasis in human cells.
An important aspect is to gain a molecular-level understanding of how cellular stress responses change membrane organelle function, protein degradation, pH and calcium regulation and signalling events including ubiquitin signalling.
The combined dynamics regulate proteostasis and is monitored by protein quality control machinery.
- Ubiquitin signalling and dynamics in proteostasis regulation and membrane trafficking in human disease.
- Protein networks that regulate cargo sorting especially at the lysosome-autophagy pathway.
- Functional interaction networks modulating proteostasis by cellular stress responses; organelle proteomics and effect of pH and calcium regulation.
|
A novel fluorescent probe reveals starvation controls the commitment of amyloid precursor protein to the lysosome. Biochim Biophys Acta. 2017 Oct;1864(10):1554-1565. doi: 10.1016/j.bbamcr.2017.06.011. Epub 2017 Jun 19. PMID: 28641977 |
A novel fluorescent probe reveals starvation controls the commitment of amyloid precursor protein to the lysosome. |
|
Leukoencephalopathy-causing CLCN2 mutations are associated with impaired Cl- channel function and trafficking. J Physiol. 2017 Sep 14. doi: 10.1113/JP275087 |
Leukoencephalopathy-causing CLCN2 mutations are associated with impaired Cl- channel function and trafficking. |
|
Chaperones rescue the energetic landscape of mutant CFTR at single molecule and in cell. Nat Commun. 2017 Aug 30;8(1):398. doi: 10.1038/s41467-017-00444-4. |
Chaperones rescue the energetic landscape of mutant CFTR at single molecule and in cell. |
|
Interplay of endosomal pH and ligand occupancy in integrin 51 ubiquitination, endocytic sorting and cell migration. Cell Rep. 2015 Oct 20;13(3):599-609. doi: 10.1016/j.celrep.2015.09.024. Epub 2015 Oct 8. |
Interplay of endosomal pH and ligand occupancy in integrin 51 ubiquitination, endocytic sorting and cell migration. |
|
Protein homeostasis at the plasma membrane. Physiology (Bethesda). 2014 Jul;29(4):265–277. doi: 10.1152/physiol.00058.2013. |
Protein homeostasis at the plasma membrane. |
|
Some gating potentiators, including VX-770, diminish ΔF508-CFTR functional expression. Sci Transl Med. 2014 Jul 23;6(246):246ra97. doi: 10.1126/scitranslmed.3008889. |
Some gating potentiators, including VX-770, diminish ΔF508-CFTR functional expression. |
|
Degradation mechanism of a Golgi-retained distal renal tubular acidosis mutant of the kidney anion exchanger 1 in renal cells. Am J Physiol Cell Physiol. 2014 Aug 1;307(3):C296-307. doi: 10.1152/ajpcell.00310.2013. Epub 2014 Jun 11. |
Degradation mechanism of a Golgi-retained distal renal tubular acidosis mutant of the kidney anion exchanger 1 in renal cells. |
|
Protein quality control at the plasma membrane. Curr Opin Cell Biol. 2011 Aug;23(4):483-91. doi: 10.1016/j.ceb.2011.04.012. Epub 2011 May 14. |
Protein quality control at the plasma membrane. |