Cell Motility and Mechanobiology

The Biro Group investigates how immune cells locate and kill cancer cells, adopting multi-disciplinary methods encompassing biophysics, cell biology, immunology, cancer biology, advanced microscopy, image analysis, and mathematical and coputational modelling. 

Biro Group 2019

From left to right: Jorge Luis Galeano Niño (PhD student), Daryan Kempe (postdoc), Manuela Ecker (PhD student, Rossy lab), Sophie Pageon (postdoc), Feyza Colakoglu (research assistant), Maté Biro (group leader), Szun Szun Tay (postdoc), Jessica Mazalo (PhD student), James Cremasco (PhD student), Satya Arjunan (postdoc), Jacqueline Tearle (PhD student), Florence Piastra-Facon (visiting student, ENS Lyon), Matt Govendir (PhD student).

  • Killer immune cell motility and search for cancer cells
  • Emergent/collective behaviour in immune cell populations
  • Mechanics of cytotoxic interactions between immune cells and tumour cells
  • Search strategy optimisation (theory and computational modelling)
  • Imaging and quantification of actin cytoskeleton dynamics
  • Mechanics of motility and cell polarisation

Highlight publications

Published In

Geoghegan N., Evelyn C., Whitehead L., Pasternak M., McDonald P., Triglia T., Marapana D., Kempe D., Thompson J., Mlodzianoski M., Healer J., Biro M., Cowman A., and Rogers K. (2021)

4D analysis of malaria parasite invasion offers insights into erythrocyte membrane remodeling and parasitophorous vacuole formation

Zhang L., Chen C., Tay S.S., Wen S., Cao C., Biro M., Jin D., Stenzel M. (2021)

Optimizing the Polymer Cloak for Upconverting Nanoparticles: An Evaluation of Bioactivity and Optical Performance

Hywood J.D., Rice G., Pageon S.V., Read M.N. and Biro M. (2021)

*Journal issue cover

Detection and characterization of chemotaxis without cell tracking

McDonald M.M., Khoo W.H., Ng P.Y., Xiao Y., Zamerli J., Thatcher P., Kyaw W., Pathmanandavel K., Grootveld A.K., Moran I., Butt D., Nguyen A., Corr A., Warren S., Biro M., Butterfield N.C., Guilfoyle S.E., Komla-Ebri D., Dack M.R.G., Dewhurst H.F., Logan J.G., Li Y., Mohanty S.T., Byrne N., Terry R.L., Simic M.K., Chai R., Quinn J.M.W., Youlten S.E., Pettitt J.A., Abi-Hanna D., Jain R., Weninger W., Lundberg M., Sun S., Ebetino H., Timpson P., Lee W.M., Baldock P.A., Rogers M.J., Brink R., Williams G.R., Bassett J.H.D., Kemp J.P., Pavlos N.J., Croucher P.I., Phan T.G. (2021)

Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption

Cell 84(5):1330-1347.E13

Lamm N., Read M.N., Nobis M., Ly D.V., Page S.G., Masamsetti V.P., Timpson P., Biro M. and Cesare A.J. (2020)

Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress

Nature Cell Biology doi: 10.1038/s41556-020-00605-6

Galeano Niño J.L, Pageon S.V., Tay S.S., Colakoglu F., Kempe D., Hywood J., Mazalo J.K., Cremasco J., Govendir M.A., Dagley L.F., Hsu K., Rizzetto S., Zieba J., Rice G., Prior V., O’Neill G.M., Williams R.J., Nisbet D.R., Kramer B., Webb A.I., Luciani F., Read M.N., Biro M. (2020)

Cytotoxic T Cells Swarm by Homotypic Chemokine Signalling

eLife 9:e56554

Arora A., Galeano Niño J.L., Myaing M.Z., Chia S., Arasi B., Ravasio A., Huang R.Y.J., Dasgupta R., Biro M., and Viasnoff V. (2020)

Two high-yield complementary methods to sort cell populations by their 2D or 3D migration speed

Molecular Biology of the Cell 31(25): 2779–2790

Lim H.Y.G., Alvarez Y.D., Gasnier M., WangY., Tetlak P., Bissiere S., Wang H., Biro M., Plachta N. (2020)

Keratins are asymmetrically inherited fate determinants in the mammalian embryo

Nature  585(7825):404-409

Piper A-K., Sophocleous R., Ross S., Evesson F., Saleh O., Bournazos A.,  Yasa J., Reed C., Woolger N., Sluyter R., Greer P., Biro M., Lemckert F., Cooper S. (2020)

Loss of calpains-1 and -2 prevents repair of plasma membrane scrape injuries, but not small pores, and induces a severe muscular dystrophy

Patkunarajah A., Stear J.H.,Moroni M., Schroeter L., Blaszkiewicz J., Tearle J.L.E., Cox C.D., Fuerst C., Sanchez-Carranza O., del Angel Ocana Fernandez M., Fleischer R., Eravci M., Weise C., Martinac B., Biro M., Lewin G.R, Poole K. (2020)

TMEM87a/Elkin1, a component of a novel mechanoelectrical transduction pathway, modulates melanoma adhesion and migration

eLife 9:e53308 

Galeano Niño J.L.*, Tay S.S*., Tearle J.L.E.*, Xie J., Govendir M.A., Kempe D., Mazalo J., Drew A.P., Colakoglu F., Kummerfeld S.K., Proud C.G., Biro M. (2020)

The Lifeact-EGFP mouse is a translationally controlled fluorescent reporter of T cell activation

Journal of Cell Science 133(5): jcs238014

Obeidy, P., Lining, J., Oehlers, S., Zulkhernain, N., Lee, Q., Galeano Niño, J., Kwan, R., Tikoo, S., Cavanagh, L., Mrass, P., Cook, A., Jackson, S., Biro, M., Roediger, B., Sixt, M., Weninger, W. (2019)

Partial loss of actin nucleator Actin Related Protein 2/3 activity triggers blebbing in primary T lymphocytes

Redpath G.M.I., Ecker M., Kapoor-Kaushik N., Vartoukian H., Carnell M., Kempe D., Biro M., Ariotti N., Rossy J. (2019)

Flotillins promote T cell receptor sorting through a fast Rab5-Rab11 endocytic recycling axis

Meiring J., Bryce N., Galeano Niño J.L., Gabriel A., Tay S.S., Hardeman E., Biro M., Gunning P. (2019)

Tropomyosin concentration but not formin nucleators mDia1 and mDia3 determines the level of tropomyosin incorporation into actin filaments

Mitchell C.B., Black B., Sun F., Chrzanowski W., Cooper-White J., Maisonneuve B., Stringer B., Day B., Biro M., O’Neill G.M. (2018)

Tropomyosin Tpm 2.1 loss induces glioblastoma spreading in soft brain-like environments

Moran I., Nguyen A., Khoo W.H., Butt D., Bourne K., Young C., Hermes J.R., Biro M., Gracie G., Ma C.S., Munier C.M.L., Luciani F., Zaunders J., Parker A., Kelleher A.D., Tangye S.G., Croucher P.I., Brink R., Read M.N., Phan T.G. (2018)

Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes

Pageon S.V., Govendir M.A., Kempe D., Biro M. (2018)

Mechanoimmunology: molecular-scale forces govern immune cell functions

Zenker J., White M.D., Gasnier M., Alvarez Y.D., Lim H.Y.G., Bissiere S., Biro M.*, Plachta N.* (2018)

(*senior and corresponding author) 

Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation

Cell 173(3):776-791.e17

Chou J., Poole K., Biro M. (2017)

Your body’s cells use and resist force, and they move. It’s mechanobiology.

The Conversation October 20, 2017

Sedzinski J., Hannezo E., Tu F., Biro M., and Wallingford J. (2017)

RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells

Journal of Cell Science 130(2): 420-428

Marshall A.D., Bailey C.G., Champ K., Vellozzi M., O’Young P., Metierre C., Feng Y., Thoeng A., Richards A.M., Schmitz U., Biro M., Jayasinghe R., Ding L., Anderson L., Mardis E.R., Rasko J.E.J. (2017)

CTCF Genetic Alterations in Endometrial Carcinoma are Pro-tumorigenic 

Oncogene 36(29):4100-4110

Newman P., Galenano Niño J.L., Graney P., Razal J.M., Minett A.I., Ribas J., Ovalle-Robles R., Biro M.*, Zreiqat H.* (2016)

(*senior and corresponding author)

Relationship between nanotopographical alignment and stem cell fate with live imaging and shape analysis 

Scientific Reports 6:37909 doi: 10.1038/srep37909

Tay SS, Carol H, Biro M. (2016)

TriKEs and BiKEs join CARs on the cancer immunotherapy highway.

Galeano Niño JL, Kwan RY, Weninger W, Biro M. (2016)

Antigen-specific T cells fully conserve antitumour function following cryopreservation.

Immunology and Cell Biology 94(4): 411-418 

Sedzinski J, Hannezo E, Tu F, Biro M, Wallingford JB. (2016)

Emergence of an apical epithelial cell surface In vivo.

Developmental Cell 36(1):24-35

Newman P, Lu Z, Roohani-Esfahani SI, Church TL, Biro M, Davies B, King A, Mackenzie K, Minett AI, Zreiqat H. (2015)

Porous and strong three-dimensional carbon nanotube coated ceramic scaffolds for tissue engineering.

Tong PL, Roediger B, Kolesnikoff N, Biro M, Tay SS, Jain R, Shaw LE, Grimbaldeston MA, Weninger W. (2015)

The skin immune atlas: three-dimensional analysis of cutaneous leukocyte subsets by in vivo microscopy

Biro M, Maître JL. (2015)

Dual pipette aspiration: a unique tool for studying intercellular adhesion.

Munoz MA*, Biro M*, Weninger W. (2014)

(*equal contribution)

T cell migration in intact lymph nodes in vivo.

Biro M*, Munoz MA, Weninger W. (2014)

(*corresponding author)

Targeting Rho-GTPases in immune cell migration and inflammation.

Weninger W, Biro M, Jain R. (2014)

Leukocyte migration in the interstitial space of non-lymphoid organs.

Bovellan M, Romeo Y, Biro M, Fritzsche M, Boden A, Moulding D, Thorogate R, Jégou A, Thrasher AJ, Romet-Lemonne G, Paluch E, Roux PP, Charras G. (2014)

Cellular control of cortical actin nucleation

Current Biology 24(14):1628-1635

Biro M, Romeo Y, Kroschwald S, Bovellan M, Boden A, Tcherkezian J, Roux PP, Charras G, Paluch E. (2013)

Cell cortex composition and homeostasis resolved by integrating proteomics and quantitative imaging.

Cytoskeleton 70(11):741-754

Sedzinski J*, Biro M*, Oswald A, Tinevez JY, Salbreux G, Paluch E. (2011)

(*equal contribution)

Polar actomyosin contractility destabilizes the position of the cytokinetic furrow.

Nature 476(7361):462-466

Puah W.C., Cheok L.P., Biro M., Ng W.T., Wasser M. (2011)

TLM-Converter: reorganization of long time-lapse microscopy datasets for downstream image analysis

BioTechniques 51(1): 49-54