Understanding the inner workings of a cell
Curious how cells are kept running like well-oiled machines, Dr Vaishnavi Ananthanarayanan uses high-resolution, live cell imaging to investigate cellular dynamics within the crowded environment inside mammalian cells. Her particular interests include how protein motors sort and transport vital cargo into and throughout cells, and how they organise subcellular compartments – like mitochondria and endosomes – to maintain cellular function.
Monitoring the movement of these motors along a network of microtubules inside cells and their coordinated interactions with these microtubule tracks, she hopes to discover how cellular organisation changes during diseases like neurodegeneration, and how protein motors like cytoplasmic dynein-1 are involved in this re-organisation.
Cells employ motor proteins to carry out a myriad of functions including maintenance of cellular organization, transport of substances across the cell, and generation of forces required for cell division. These activities of motor proteins are facilitated by self-organising polymers inside the cell, including the microtubules. Microtubules function as tracks for motor movement, and alternately as ropes, which motor proteins pull on. We have gained a wealth of information by replicating cellular processes involving motors and the cytoskeleton outside living cells, i.e. ‘in vitro’. However, understanding the complex intracellular milieu within which motors operate to organise the cell remains an elusive quest. A thorough investigation of processes regulating motors and microtubules therefore lets us see how these processes unravel in contexts of both health and disease (including neurodegeneration and cancers), where they go rogue.
The Ananthanarayanan Group aims to investigate cellular organisation brought about by the activity of motor proteins and the cytoskeleton across scales: from single molecules to whole organelles, by employing advanced live-cell microscopy techniques with high spatial and temporal resolution.
Understanding how stochastic and rare events, such as motor protein binding to cytoskeletal tracks or cargo, give rise to complex cellular organisation across scales.
- Regulation of motor proteins
- Cytoskeleton-organelle interactions
- Intracellular organisation
- Cellular decision making
- In vivo single-molecule imaging
J. Cell Biol. (2019) 218 (11) 3560-3571.
|Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance.|
J. Biol. Chem. (2019) 294(10) 3385–3396.
|Association of mitochondria with microtubules inhibits mitochondrial fission by precluding activity of the fission protein Dnm1.|
Proc. Nat. Acad. Sci. (2017) 114: E2672-E2681.
|Fission Yeast Myosin I Facilitates PI(4,5)P2 –mediated Anchoring of Cytoplasmic Dynein to the Cortex.|
ACS Biomater. (2017) Sci. Eng. 3: 551-559.
|Role of Microtubules in Osteogenic Differentiation of Mesenchymal Stem Cells on 3D Nanofibrous Scaffolds.|
Cell (2013) 153: 1526-1536.
|Dynein Motion Switches from Diffusive to Directed upon Cortical Anchoring.|